fluorometric assay kit Search Results


caspase 3 assay kit  (R&D Systems)
91
R&D Systems caspase 3 assay kit
Caspase 3 Assay Kit, supplied by R&D Systems, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/caspase 3 assay kit/product/R&D Systems
Average 91 stars, based on 1 article reviews
caspase 3 assay kit - by Bioz Stars, 2026-03
91/100 stars
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ctsb  (Novus Biologicals)
93
Novus Biologicals ctsb
A MIA PaCa-2 cells were incubated for 24 h with vehicle (Control) <t>or</t> <t>gemcitabine</t> (10 µM) before lysosome labeling using anti-LAMP1 antibody. Nuclei were stained with DAPI. Scale bars , 10 µm. Graph shows numbers of LAMP1 puncta per cell calculated using CellProfiler software. Scatter dot plot shows data as means ± SD; n = 31‒48 cells from three independent experiments. Data were statistically analyzed using unpaired t-tests. B Representative images of live MIA PaCa-2 cells incubated with vehicle (Control) or gemcitabine (10 µM) for 24 h then stained with LysoTracker. C Representative images of live MIA PaCa-2 cells incubated with vehicle (Control) or gemcitabine (10 µM) for 24 h then stained with Magic Red. D HeLa cells were incubated for 24 h with vehicle (Control) or gemcitabine (10 µM) before lysosome labeling using anti-LAMP1 antibody. Nuclei were stained with DAPI. Scale bars , 10 µm. Graph shows numbers of LAMP1 puncta per cell calculated using CellProfiler software. Scatter dot plot shows data as means ± SD; n = 22–28 cells from three independent experiments. Data were statistically analyzed using unpaired t -tests. E Representative images of live HeLa cells incubated with vehicle (Control) or gemcitabine (10 µM) for 24 h then stained with Lysotracker. F Representative images of live HeLa cells incubated with vehicle (Control) or gemcitabine (10 µM) for 24 h then stained with Magic Red. G MIA PaCa-2 cells were incubated with vehicle (−) or gemcitabine (10 µM) for the indicated periods. Total cell lysates were analyzed by immunoblotted using <t>anti-CTSB</t> and β-ACTIN antibodies. Levels of pro-CTSB are barely detectable compared with mature CTSB.
Ctsb, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/ctsb/product/Novus Biologicals
Average 93 stars, based on 1 article reviews
ctsb - by Bioz Stars, 2026-03
93/100 stars
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calcein am cell viability assay kit  (Trevigen)
93
Trevigen calcein am cell viability assay kit
A MIA PaCa-2 cells were incubated for 24 h with vehicle (Control) <t>or</t> <t>gemcitabine</t> (10 µM) before lysosome labeling using anti-LAMP1 antibody. Nuclei were stained with DAPI. Scale bars , 10 µm. Graph shows numbers of LAMP1 puncta per cell calculated using CellProfiler software. Scatter dot plot shows data as means ± SD; n = 31‒48 cells from three independent experiments. Data were statistically analyzed using unpaired t-tests. B Representative images of live MIA PaCa-2 cells incubated with vehicle (Control) or gemcitabine (10 µM) for 24 h then stained with LysoTracker. C Representative images of live MIA PaCa-2 cells incubated with vehicle (Control) or gemcitabine (10 µM) for 24 h then stained with Magic Red. D HeLa cells were incubated for 24 h with vehicle (Control) or gemcitabine (10 µM) before lysosome labeling using anti-LAMP1 antibody. Nuclei were stained with DAPI. Scale bars , 10 µm. Graph shows numbers of LAMP1 puncta per cell calculated using CellProfiler software. Scatter dot plot shows data as means ± SD; n = 22–28 cells from three independent experiments. Data were statistically analyzed using unpaired t -tests. E Representative images of live HeLa cells incubated with vehicle (Control) or gemcitabine (10 µM) for 24 h then stained with Lysotracker. F Representative images of live HeLa cells incubated with vehicle (Control) or gemcitabine (10 µM) for 24 h then stained with Magic Red. G MIA PaCa-2 cells were incubated with vehicle (−) or gemcitabine (10 µM) for the indicated periods. Total cell lysates were analyzed by immunoblotted using <t>anti-CTSB</t> and β-ACTIN antibodies. Levels of pro-CTSB are barely detectable compared with mature CTSB.
Calcein Am Cell Viability Assay Kit, supplied by Trevigen, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/calcein am cell viability assay kit/product/Trevigen
Average 93 stars, based on 1 article reviews
calcein am cell viability assay kit - by Bioz Stars, 2026-03
93/100 stars
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fluorometric caspase assay kit  (R&D Systems)
91
R&D Systems fluorometric caspase assay kit
A MIA PaCa-2 cells were incubated for 24 h with vehicle (Control) <t>or</t> <t>gemcitabine</t> (10 µM) before lysosome labeling using anti-LAMP1 antibody. Nuclei were stained with DAPI. Scale bars , 10 µm. Graph shows numbers of LAMP1 puncta per cell calculated using CellProfiler software. Scatter dot plot shows data as means ± SD; n = 31‒48 cells from three independent experiments. Data were statistically analyzed using unpaired t-tests. B Representative images of live MIA PaCa-2 cells incubated with vehicle (Control) or gemcitabine (10 µM) for 24 h then stained with LysoTracker. C Representative images of live MIA PaCa-2 cells incubated with vehicle (Control) or gemcitabine (10 µM) for 24 h then stained with Magic Red. D HeLa cells were incubated for 24 h with vehicle (Control) or gemcitabine (10 µM) before lysosome labeling using anti-LAMP1 antibody. Nuclei were stained with DAPI. Scale bars , 10 µm. Graph shows numbers of LAMP1 puncta per cell calculated using CellProfiler software. Scatter dot plot shows data as means ± SD; n = 22–28 cells from three independent experiments. Data were statistically analyzed using unpaired t -tests. E Representative images of live HeLa cells incubated with vehicle (Control) or gemcitabine (10 µM) for 24 h then stained with Lysotracker. F Representative images of live HeLa cells incubated with vehicle (Control) or gemcitabine (10 µM) for 24 h then stained with Magic Red. G MIA PaCa-2 cells were incubated with vehicle (−) or gemcitabine (10 µM) for the indicated periods. Total cell lysates were analyzed by immunoblotted using <t>anti-CTSB</t> and β-ACTIN antibodies. Levels of pro-CTSB are barely detectable compared with mature CTSB.
Fluorometric Caspase Assay Kit, supplied by R&D Systems, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/fluorometric caspase assay kit/product/R&D Systems
Average 91 stars, based on 1 article reviews
fluorometric caspase assay kit - by Bioz Stars, 2026-03
91/100 stars
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cathepsin l assay kit  (Novus Biologicals)
92
Novus Biologicals cathepsin l assay kit
A MIA PaCa-2 cells were incubated for 24 h with vehicle (Control) <t>or</t> <t>gemcitabine</t> (10 µM) before lysosome labeling using anti-LAMP1 antibody. Nuclei were stained with DAPI. Scale bars , 10 µm. Graph shows numbers of LAMP1 puncta per cell calculated using CellProfiler software. Scatter dot plot shows data as means ± SD; n = 31‒48 cells from three independent experiments. Data were statistically analyzed using unpaired t-tests. B Representative images of live MIA PaCa-2 cells incubated with vehicle (Control) or gemcitabine (10 µM) for 24 h then stained with LysoTracker. C Representative images of live MIA PaCa-2 cells incubated with vehicle (Control) or gemcitabine (10 µM) for 24 h then stained with Magic Red. D HeLa cells were incubated for 24 h with vehicle (Control) or gemcitabine (10 µM) before lysosome labeling using anti-LAMP1 antibody. Nuclei were stained with DAPI. Scale bars , 10 µm. Graph shows numbers of LAMP1 puncta per cell calculated using CellProfiler software. Scatter dot plot shows data as means ± SD; n = 22–28 cells from three independent experiments. Data were statistically analyzed using unpaired t -tests. E Representative images of live HeLa cells incubated with vehicle (Control) or gemcitabine (10 µM) for 24 h then stained with Lysotracker. F Representative images of live HeLa cells incubated with vehicle (Control) or gemcitabine (10 µM) for 24 h then stained with Magic Red. G MIA PaCa-2 cells were incubated with vehicle (−) or gemcitabine (10 µM) for the indicated periods. Total cell lysates were analyzed by immunoblotted using <t>anti-CTSB</t> and β-ACTIN antibodies. Levels of pro-CTSB are barely detectable compared with mature CTSB.
Cathepsin L Assay Kit, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/cathepsin l assay kit/product/Novus Biologicals
Average 92 stars, based on 1 article reviews
cathepsin l assay kit - by Bioz Stars, 2026-03
92/100 stars
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viability assay kit  (R&D Systems)
93
R&D Systems viability assay kit
A MIA PaCa-2 cells were incubated for 24 h with vehicle (Control) <t>or</t> <t>gemcitabine</t> (10 µM) before lysosome labeling using anti-LAMP1 antibody. Nuclei were stained with DAPI. Scale bars , 10 µm. Graph shows numbers of LAMP1 puncta per cell calculated using CellProfiler software. Scatter dot plot shows data as means ± SD; n = 31‒48 cells from three independent experiments. Data were statistically analyzed using unpaired t-tests. B Representative images of live MIA PaCa-2 cells incubated with vehicle (Control) or gemcitabine (10 µM) for 24 h then stained with LysoTracker. C Representative images of live MIA PaCa-2 cells incubated with vehicle (Control) or gemcitabine (10 µM) for 24 h then stained with Magic Red. D HeLa cells were incubated for 24 h with vehicle (Control) or gemcitabine (10 µM) before lysosome labeling using anti-LAMP1 antibody. Nuclei were stained with DAPI. Scale bars , 10 µm. Graph shows numbers of LAMP1 puncta per cell calculated using CellProfiler software. Scatter dot plot shows data as means ± SD; n = 22–28 cells from three independent experiments. Data were statistically analyzed using unpaired t -tests. E Representative images of live HeLa cells incubated with vehicle (Control) or gemcitabine (10 µM) for 24 h then stained with Lysotracker. F Representative images of live HeLa cells incubated with vehicle (Control) or gemcitabine (10 µM) for 24 h then stained with Magic Red. G MIA PaCa-2 cells were incubated with vehicle (−) or gemcitabine (10 µM) for the indicated periods. Total cell lysates were analyzed by immunoblotted using <t>anti-CTSB</t> and β-ACTIN antibodies. Levels of pro-CTSB are barely detectable compared with mature CTSB.
Viability Assay Kit, supplied by R&D Systems, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/viability assay kit/product/R&D Systems
Average 93 stars, based on 1 article reviews
viability assay kit - by Bioz Stars, 2026-03
93/100 stars
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caspase 3 devd r110 fluorometric  (Biotium)
93
Biotium caspase 3 devd r110 fluorometric
A MIA PaCa-2 cells were incubated for 24 h with vehicle (Control) <t>or</t> <t>gemcitabine</t> (10 µM) before lysosome labeling using anti-LAMP1 antibody. Nuclei were stained with DAPI. Scale bars , 10 µm. Graph shows numbers of LAMP1 puncta per cell calculated using CellProfiler software. Scatter dot plot shows data as means ± SD; n = 31‒48 cells from three independent experiments. Data were statistically analyzed using unpaired t-tests. B Representative images of live MIA PaCa-2 cells incubated with vehicle (Control) or gemcitabine (10 µM) for 24 h then stained with LysoTracker. C Representative images of live MIA PaCa-2 cells incubated with vehicle (Control) or gemcitabine (10 µM) for 24 h then stained with Magic Red. D HeLa cells were incubated for 24 h with vehicle (Control) or gemcitabine (10 µM) before lysosome labeling using anti-LAMP1 antibody. Nuclei were stained with DAPI. Scale bars , 10 µm. Graph shows numbers of LAMP1 puncta per cell calculated using CellProfiler software. Scatter dot plot shows data as means ± SD; n = 22–28 cells from three independent experiments. Data were statistically analyzed using unpaired t -tests. E Representative images of live HeLa cells incubated with vehicle (Control) or gemcitabine (10 µM) for 24 h then stained with Lysotracker. F Representative images of live HeLa cells incubated with vehicle (Control) or gemcitabine (10 µM) for 24 h then stained with Magic Red. G MIA PaCa-2 cells were incubated with vehicle (−) or gemcitabine (10 µM) for the indicated periods. Total cell lysates were analyzed by immunoblotted using <t>anti-CTSB</t> and β-ACTIN antibodies. Levels of pro-CTSB are barely detectable compared with mature CTSB.
Caspase 3 Devd R110 Fluorometric, supplied by Biotium, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/caspase 3 devd r110 fluorometric/product/Biotium
Average 93 stars, based on 1 article reviews
caspase 3 devd r110 fluorometric - by Bioz Stars, 2026-03
93/100 stars
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fluorometric assay kit  (R&D Systems)
90
R&D Systems fluorometric assay kit
A MIA PaCa-2 cells were incubated for 24 h with vehicle (Control) <t>or</t> <t>gemcitabine</t> (10 µM) before lysosome labeling using anti-LAMP1 antibody. Nuclei were stained with DAPI. Scale bars , 10 µm. Graph shows numbers of LAMP1 puncta per cell calculated using CellProfiler software. Scatter dot plot shows data as means ± SD; n = 31‒48 cells from three independent experiments. Data were statistically analyzed using unpaired t-tests. B Representative images of live MIA PaCa-2 cells incubated with vehicle (Control) or gemcitabine (10 µM) for 24 h then stained with LysoTracker. C Representative images of live MIA PaCa-2 cells incubated with vehicle (Control) or gemcitabine (10 µM) for 24 h then stained with Magic Red. D HeLa cells were incubated for 24 h with vehicle (Control) or gemcitabine (10 µM) before lysosome labeling using anti-LAMP1 antibody. Nuclei were stained with DAPI. Scale bars , 10 µm. Graph shows numbers of LAMP1 puncta per cell calculated using CellProfiler software. Scatter dot plot shows data as means ± SD; n = 22–28 cells from three independent experiments. Data were statistically analyzed using unpaired t -tests. E Representative images of live HeLa cells incubated with vehicle (Control) or gemcitabine (10 µM) for 24 h then stained with Lysotracker. F Representative images of live HeLa cells incubated with vehicle (Control) or gemcitabine (10 µM) for 24 h then stained with Magic Red. G MIA PaCa-2 cells were incubated with vehicle (−) or gemcitabine (10 µM) for the indicated periods. Total cell lysates were analyzed by immunoblotted using <t>anti-CTSB</t> and β-ACTIN antibodies. Levels of pro-CTSB are barely detectable compared with mature CTSB.
Fluorometric Assay Kit, supplied by R&D Systems, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/fluorometric assay kit/product/R&D Systems
Average 90 stars, based on 1 article reviews
fluorometric assay kit - by Bioz Stars, 2026-03
90/100 stars
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cell proliferation  (R&D Systems)
90
R&D Systems cell proliferation
A MIA PaCa-2 cells were incubated for 24 h with vehicle (Control) <t>or</t> <t>gemcitabine</t> (10 µM) before lysosome labeling using anti-LAMP1 antibody. Nuclei were stained with DAPI. Scale bars , 10 µm. Graph shows numbers of LAMP1 puncta per cell calculated using CellProfiler software. Scatter dot plot shows data as means ± SD; n = 31‒48 cells from three independent experiments. Data were statistically analyzed using unpaired t-tests. B Representative images of live MIA PaCa-2 cells incubated with vehicle (Control) or gemcitabine (10 µM) for 24 h then stained with LysoTracker. C Representative images of live MIA PaCa-2 cells incubated with vehicle (Control) or gemcitabine (10 µM) for 24 h then stained with Magic Red. D HeLa cells were incubated for 24 h with vehicle (Control) or gemcitabine (10 µM) before lysosome labeling using anti-LAMP1 antibody. Nuclei were stained with DAPI. Scale bars , 10 µm. Graph shows numbers of LAMP1 puncta per cell calculated using CellProfiler software. Scatter dot plot shows data as means ± SD; n = 22–28 cells from three independent experiments. Data were statistically analyzed using unpaired t -tests. E Representative images of live HeLa cells incubated with vehicle (Control) or gemcitabine (10 µM) for 24 h then stained with Lysotracker. F Representative images of live HeLa cells incubated with vehicle (Control) or gemcitabine (10 µM) for 24 h then stained with Magic Red. G MIA PaCa-2 cells were incubated with vehicle (−) or gemcitabine (10 µM) for the indicated periods. Total cell lysates were analyzed by immunoblotted using <t>anti-CTSB</t> and β-ACTIN antibodies. Levels of pro-CTSB are barely detectable compared with mature CTSB.
Cell Proliferation, supplied by R&D Systems, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/cell proliferation/product/R&D Systems
Average 90 stars, based on 1 article reviews
cell proliferation - by Bioz Stars, 2026-03
90/100 stars
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caspase 1 assay kit  (R&D Systems)
93
R&D Systems caspase 1 assay kit
A MIA PaCa-2 cells were incubated for 24 h with vehicle (Control) <t>or</t> <t>gemcitabine</t> (10 µM) before lysosome labeling using anti-LAMP1 antibody. Nuclei were stained with DAPI. Scale bars , 10 µm. Graph shows numbers of LAMP1 puncta per cell calculated using CellProfiler software. Scatter dot plot shows data as means ± SD; n = 31‒48 cells from three independent experiments. Data were statistically analyzed using unpaired t-tests. B Representative images of live MIA PaCa-2 cells incubated with vehicle (Control) or gemcitabine (10 µM) for 24 h then stained with LysoTracker. C Representative images of live MIA PaCa-2 cells incubated with vehicle (Control) or gemcitabine (10 µM) for 24 h then stained with Magic Red. D HeLa cells were incubated for 24 h with vehicle (Control) or gemcitabine (10 µM) before lysosome labeling using anti-LAMP1 antibody. Nuclei were stained with DAPI. Scale bars , 10 µm. Graph shows numbers of LAMP1 puncta per cell calculated using CellProfiler software. Scatter dot plot shows data as means ± SD; n = 22–28 cells from three independent experiments. Data were statistically analyzed using unpaired t -tests. E Representative images of live HeLa cells incubated with vehicle (Control) or gemcitabine (10 µM) for 24 h then stained with Lysotracker. F Representative images of live HeLa cells incubated with vehicle (Control) or gemcitabine (10 µM) for 24 h then stained with Magic Red. G MIA PaCa-2 cells were incubated with vehicle (−) or gemcitabine (10 µM) for the indicated periods. Total cell lysates were analyzed by immunoblotted using <t>anti-CTSB</t> and β-ACTIN antibodies. Levels of pro-CTSB are barely detectable compared with mature CTSB.
Caspase 1 Assay Kit, supplied by R&D Systems, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/caspase 1 assay kit/product/R&D Systems
Average 93 stars, based on 1 article reviews
caspase 1 assay kit - by Bioz Stars, 2026-03
93/100 stars
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fmk 2201 nb  (Novus Biologicals)
92
Novus Biologicals fmk 2201 nb
A MIA PaCa-2 cells were incubated for 24 h with vehicle (Control) <t>or</t> <t>gemcitabine</t> (10 µM) before lysosome labeling using anti-LAMP1 antibody. Nuclei were stained with DAPI. Scale bars , 10 µm. Graph shows numbers of LAMP1 puncta per cell calculated using CellProfiler software. Scatter dot plot shows data as means ± SD; n = 31‒48 cells from three independent experiments. Data were statistically analyzed using unpaired t-tests. B Representative images of live MIA PaCa-2 cells incubated with vehicle (Control) or gemcitabine (10 µM) for 24 h then stained with LysoTracker. C Representative images of live MIA PaCa-2 cells incubated with vehicle (Control) or gemcitabine (10 µM) for 24 h then stained with Magic Red. D HeLa cells were incubated for 24 h with vehicle (Control) or gemcitabine (10 µM) before lysosome labeling using anti-LAMP1 antibody. Nuclei were stained with DAPI. Scale bars , 10 µm. Graph shows numbers of LAMP1 puncta per cell calculated using CellProfiler software. Scatter dot plot shows data as means ± SD; n = 22–28 cells from three independent experiments. Data were statistically analyzed using unpaired t -tests. E Representative images of live HeLa cells incubated with vehicle (Control) or gemcitabine (10 µM) for 24 h then stained with Lysotracker. F Representative images of live HeLa cells incubated with vehicle (Control) or gemcitabine (10 µM) for 24 h then stained with Magic Red. G MIA PaCa-2 cells were incubated with vehicle (−) or gemcitabine (10 µM) for the indicated periods. Total cell lysates were analyzed by immunoblotted using <t>anti-CTSB</t> and β-ACTIN antibodies. Levels of pro-CTSB are barely detectable compared with mature CTSB.
Fmk 2201 Nb, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/fmk 2201 nb/product/Novus Biologicals
Average 92 stars, based on 1 article reviews
fmk 2201 nb - by Bioz Stars, 2026-03
92/100 stars
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Image Search Results


A MIA PaCa-2 cells were incubated for 24 h with vehicle (Control) or gemcitabine (10 µM) before lysosome labeling using anti-LAMP1 antibody. Nuclei were stained with DAPI. Scale bars , 10 µm. Graph shows numbers of LAMP1 puncta per cell calculated using CellProfiler software. Scatter dot plot shows data as means ± SD; n = 31‒48 cells from three independent experiments. Data were statistically analyzed using unpaired t-tests. B Representative images of live MIA PaCa-2 cells incubated with vehicle (Control) or gemcitabine (10 µM) for 24 h then stained with LysoTracker. C Representative images of live MIA PaCa-2 cells incubated with vehicle (Control) or gemcitabine (10 µM) for 24 h then stained with Magic Red. D HeLa cells were incubated for 24 h with vehicle (Control) or gemcitabine (10 µM) before lysosome labeling using anti-LAMP1 antibody. Nuclei were stained with DAPI. Scale bars , 10 µm. Graph shows numbers of LAMP1 puncta per cell calculated using CellProfiler software. Scatter dot plot shows data as means ± SD; n = 22–28 cells from three independent experiments. Data were statistically analyzed using unpaired t -tests. E Representative images of live HeLa cells incubated with vehicle (Control) or gemcitabine (10 µM) for 24 h then stained with Lysotracker. F Representative images of live HeLa cells incubated with vehicle (Control) or gemcitabine (10 µM) for 24 h then stained with Magic Red. G MIA PaCa-2 cells were incubated with vehicle (−) or gemcitabine (10 µM) for the indicated periods. Total cell lysates were analyzed by immunoblotted using anti-CTSB and β-ACTIN antibodies. Levels of pro-CTSB are barely detectable compared with mature CTSB.

Journal: Cell Death Discovery

Article Title: Gemcitabine promotes autophagy and lysosomal function through ERK- and TFEB-dependent mechanisms

doi: 10.1038/s41420-023-01342-z

Figure Lengend Snippet: A MIA PaCa-2 cells were incubated for 24 h with vehicle (Control) or gemcitabine (10 µM) before lysosome labeling using anti-LAMP1 antibody. Nuclei were stained with DAPI. Scale bars , 10 µm. Graph shows numbers of LAMP1 puncta per cell calculated using CellProfiler software. Scatter dot plot shows data as means ± SD; n = 31‒48 cells from three independent experiments. Data were statistically analyzed using unpaired t-tests. B Representative images of live MIA PaCa-2 cells incubated with vehicle (Control) or gemcitabine (10 µM) for 24 h then stained with LysoTracker. C Representative images of live MIA PaCa-2 cells incubated with vehicle (Control) or gemcitabine (10 µM) for 24 h then stained with Magic Red. D HeLa cells were incubated for 24 h with vehicle (Control) or gemcitabine (10 µM) before lysosome labeling using anti-LAMP1 antibody. Nuclei were stained with DAPI. Scale bars , 10 µm. Graph shows numbers of LAMP1 puncta per cell calculated using CellProfiler software. Scatter dot plot shows data as means ± SD; n = 22–28 cells from three independent experiments. Data were statistically analyzed using unpaired t -tests. E Representative images of live HeLa cells incubated with vehicle (Control) or gemcitabine (10 µM) for 24 h then stained with Lysotracker. F Representative images of live HeLa cells incubated with vehicle (Control) or gemcitabine (10 µM) for 24 h then stained with Magic Red. G MIA PaCa-2 cells were incubated with vehicle (−) or gemcitabine (10 µM) for the indicated periods. Total cell lysates were analyzed by immunoblotted using anti-CTSB and β-ACTIN antibodies. Levels of pro-CTSB are barely detectable compared with mature CTSB.

Article Snippet: MIA shNT and MIA shTFEB cells (1 × 10 5 /well) were seeded in duplicate in 12-well plates for 24 h, then incubated with vehicle or 10 μM gemcitabine for 24 h. The activity of CTSB was determined using fluorometric assays (NBP2-54841; Novus Biologicals, LLC, Centennial, CO, USA) as described by the manufacturer.

Techniques: Incubation, Control, Labeling, Staining, Software

A Quantification of MIA shNT and MIA shTFEB cells incubated for 24 h with vehicle (Control) or gemcitabine (Gem; 10 µM) and stained with LysoTracker. Data are shown as means ± SD of n = 471–2196 cells from three independent experiments and were statistically analyzed using two-way ANOVA with Tukey post hoc tests. B Quantification of MIA shNT and MIA shTFEB cells incubated for 24 h with vehicle (Control) or gemcitabine (Gem; 10 µM) and stained with Magic Red. Data are shown as means ± SD of n = 1220–3156 cells from three independent experiments and were statistically analyzed using two-way ANOVA with Tukey post hoc tests. C Activity of CTSB in MIA shNT and MIA shTFEB cells incubated with vehicle (Control) or gemcitabine (Gem; 10 µM) for 24 h ( N = 6 from three color-coded independent experiments). Data show color-coded independent experiments, means ± SD and were statistically analyzed using two-way ANOVA with Tukey post hoc tests. D MIA shNT and MIA shTFEB cells were incubated for 48 h with vehicle (Control) or gemcitabine (10 µM). Total cell lysates were analyzed by immunoblotting using anti-TFEB, CTSB, and GAPDH antibodies.

Journal: Cell Death Discovery

Article Title: Gemcitabine promotes autophagy and lysosomal function through ERK- and TFEB-dependent mechanisms

doi: 10.1038/s41420-023-01342-z

Figure Lengend Snippet: A Quantification of MIA shNT and MIA shTFEB cells incubated for 24 h with vehicle (Control) or gemcitabine (Gem; 10 µM) and stained with LysoTracker. Data are shown as means ± SD of n = 471–2196 cells from three independent experiments and were statistically analyzed using two-way ANOVA with Tukey post hoc tests. B Quantification of MIA shNT and MIA shTFEB cells incubated for 24 h with vehicle (Control) or gemcitabine (Gem; 10 µM) and stained with Magic Red. Data are shown as means ± SD of n = 1220–3156 cells from three independent experiments and were statistically analyzed using two-way ANOVA with Tukey post hoc tests. C Activity of CTSB in MIA shNT and MIA shTFEB cells incubated with vehicle (Control) or gemcitabine (Gem; 10 µM) for 24 h ( N = 6 from three color-coded independent experiments). Data show color-coded independent experiments, means ± SD and were statistically analyzed using two-way ANOVA with Tukey post hoc tests. D MIA shNT and MIA shTFEB cells were incubated for 48 h with vehicle (Control) or gemcitabine (10 µM). Total cell lysates were analyzed by immunoblotting using anti-TFEB, CTSB, and GAPDH antibodies.

Article Snippet: MIA shNT and MIA shTFEB cells (1 × 10 5 /well) were seeded in duplicate in 12-well plates for 24 h, then incubated with vehicle or 10 μM gemcitabine for 24 h. The activity of CTSB was determined using fluorometric assays (NBP2-54841; Novus Biologicals, LLC, Centennial, CO, USA) as described by the manufacturer.

Techniques: Incubation, Control, Staining, Activity Assay, Western Blot